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Mouse Fatty acid-binding protein, adipocyte(FABP4) ELISA kit

  • 中文名: 小鼠脂肪酸结合蛋白,脂肪细胞(FABP4)ELISA kit
货号: IPD-EL007945MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠脂肪酸结合蛋白,脂肪细胞(FABP4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FABP4含量。FABP4是一种脂肪酸结合蛋白,在脂质代谢、炎症反应等生理过程中发挥重要作用。研究发现它与肥胖、糖尿病、心血管疾病等密切相关。其作用机制涉及调节脂肪酸的摄取、转运和代谢,影响细胞内信号传导,为相关疾病治疗提供了新靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,该产品可广泛应用于基础科研领域,如探究代谢性疾病动物模型中脂肪细胞功能变化、评估药物或基因干预对脂代谢的影响,以及体外细胞实验中FABP4表达调控机制研究等,为代谢相关分子机制探索提供精准检测工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Fabp4 ELISA Kit; Ap2 ELISA Kit; Fatty acid-binding protein ELISA Kit; adipocyte ELISA Kit; 3T3-L1 lipid-binding protein ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; ALBP ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; A-FABP ELISA Kit; AFABP ELISA Kit; Fatty acid-binding protein 4 ELISA Kit; Myelin P2 protein homolog ELISA Kit; P15 ELISA Kit; P2 adipocyte protein ELISA Kit; Protein 422 ELISA Kit
  • 缩写:
    FABP4
  • Uniprot No.:
    P04117
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.81 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:400

    Average %

    96


    Range %

    90-100


    1:800

    Average %

    98


    Range %

    93-103


    1:1600

    Average %

    92


    Range %

    85-98


    1:3200

    Average %

    103


    Range %

    98-107


  • 回收率:

    The recovery of mouse FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    98-108


    EDTA plasma (n=4)

    96

    90-102


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.858

    2.764

    2.811

    2.670


    1000

    2.131

    2.067

    2.099

    1.958


    500

    1.275

    1.328

    1.302

    1.160


    250

    0.837

    0.829

    0.833

    0.692


    125

    0.492

    0.511

    0.501

    0.360


    62.5

    0.317

    0.333

    0.325

    0.184


    31.25

    0.238

    0.244

    0.241

    0.100


    0

    0.137

    0.145

    0.141




  • 货期:
    3-5 working days

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