首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人FADD蛋白(FADD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的FADD含量。FADD（Fas-Associated Death Domain）是一种接头蛋白，介导细胞程序性凋亡的关键蛋白。位于染色体11q13区域，常在头颈癌中扩增。研究显示，FADD通过其C端死亡结构域与TNFRSF6/Fas受体等相互作用，介导细胞凋亡信号。此外，FADD在细胞迁移、脂代谢调控等方面也具有重要作用。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于体外实验中凋亡机制研究、免疫相关疾病模型分析、药物靶点筛选及细胞信号转导研究，为探索FADD在生理病理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FADD ELISA Kit; FADD protein ELISA Kit; FADD_HUMAN ELISA Kit; Fas (TNFRSF6) associated via death domain ELISA Kit; Fas associated via death domain ELISA Kit; Fas associating death domain containing protein ELISA Kit; Fas associating protein ELISA Kit; Fas associating protein with death domain ELISA Kit; Fas TNFRSF6 associated via death domain ELISA Kit; FAS-associated death domain protein ELISA Kit; FAS-associating death domain-containing protein ELISA Kit; GIG 3 ELISA Kit; GIG3 ELISA Kit; Growth inhibiting gene 3 protein ELISA Kit; Growth-inhibiting gene 3 protein ELISA Kit; H sapiens mRNA for mediator of receptor induced toxicity ELISA Kit; Mediator of receptor induced toxicity ELISA Kit; MGC8528 ELISA Kit; MORT 1 ELISA Kit; MORT1 ELISA Kit; Protein FADD ELISA Kit
缩写：FADD
Uniprot No.：Q13158
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human FADD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of Human FADD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-94 | ||||
| EDTA plasma (n=4) | 98 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.7032.8222.7632.65940002.3282.2082.2682.16420001.6151.5341.5751.47110000.8840.9140.8990.7955000.4330.4680.4510.3472500.2810.2940.2880.1841250.1820.1860.1840.08000.1040.1030.104 | ||||||
货期：3-5 working days
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