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产品描述：人FAM13A蛋白(FAM13A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FAM13A含量。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于体外研究FAM13A在疾病模型中的表达变化、分子机制探索及生物标志物筛选，如细胞实验后的培养上清液检测、动物模型组织样本分析或临床前研究中的体液样本定量，为呼吸道疾病、代谢综合征等领域的科研工作提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FAM13A ELISA Kit; FAM13A1 ELISA Kit; KIAA0914Protein FAM13A ELISA Kit
缩写：FAM13A
Uniprot No.：O94988
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FAM13A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 88-105 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 97-108 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 88-99 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 94-108 | ||||||
回收率：
| The recovery of human FAM13A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 94-104 | |||||
| EDTA plasma (n=4) | 102 | 94-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.4672.4022.4352.2997501.6991.6191.6591.5233751.0451.0731.0590.923187.50.5850.5460.5660.43093.750.3780.3550.3670.23146.880.2380.2310.2350.09923.440.2130.2200.2170.08100.1380.1340.136 | |||||||
货期：3-5 working days
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