首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凋亡相关因子配体(FASL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FASLG含量。FASLG即Fas配体，是肿瘤坏死因子超家族成员，在免疫调节、细胞凋亡中发挥关键作用。其背景在于它参与免疫豁免和免疫逃逸机制。研究机制主要聚焦于与Fas受体结合，激活细胞内凋亡信号通路，诱导靶细胞凋亡，在肿瘤免疫治疗等领域受关注。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本试剂盒适用于科研领域，可用于探索FASL在细胞程序性死亡机制、免疫细胞功能调控、疾病模型构建（如炎症性疾病、肿瘤微环境研究）中的表达动态，为分子机制研究提供高效工具。FASL在免疫调节、免疫耐受及病理状态（如自身免疫疾病、肿瘤免疫逃逸）中发挥重要作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Faslg; Apt1lg1; Cd95l; Fasl; gld; Tnfsf6; Tumor necrosis factor ligand superfamily member 6; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD antigen CD178
缩写：FASLG
Uniprot No.：P41047
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FASL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-105 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 84-91 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
回收率：
| The recovery of mouse FASL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-94 | |||||
| EDTA plasma (n=4) | 93 | 90-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5002.4112.4312.4212.2342501.8461.8011.8241.6371251.1811.2011.1911.00462.50.8240.8310.8280.64131.20.5310.5620.5470.36015.60.3580.3480.3530.1667.80.2710.2800.2760.08900.1880.1860.187 | |||||||
货期：3-5 working days
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