首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人F框特有蛋白32(FBXO32)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FBXO32含量。FBXO32是一种重要靶点。它参与多种细胞进程和生理调节，如骨骼肌萎缩等。研究通过解析其结构和功能，揭示它在泛素-蛋白酶体系统中的作用机制，为相关疾病如肌肉减少症、肌萎缩侧索硬化症等治疗药物研发提供理论基础。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于基础科研中探索FBXO32在疾病模型中的表达调控、信号通路互作研究，或作为药物开发过程中靶点验证的工具，为肌肉代谢、癌症生物学等领域的体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：4833442G10Rik ELISA Kit; AI430017 ELISA Kit; Atrogin 1 ELISA Kit; Atrogin-1 ELISA Kit; ATROGIN1 ELISA Kit; Atrophy gene 1 ELISA Kit; F box only protein 32 ELISA Kit; F-box only protein 32 ELISA Kit; F-box protein 32 ELISA Kit; FBX32_HUMAN ELISA Kit; fbxo25 ELISA Kit; FBXO32 ELISA Kit; FLJ32424 ELISA Kit; MAFbx ELISA Kit; MGC108443 ELISA Kit; MGC137646 ELISA Kit; MGC33610 ELISA Kit; Muscle atrophy F box ELISA Kit; Muscle atrophy F box protein ELISA Kit; Muscle atrophy F-box protein ELISA Kit
缩写：FBXO32
Uniprot No.：Q969P5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FBXO32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-94 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of human FBXO32 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-96 | ||||
| EDTA plasma (n=4) | 94 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.6922.6452.6692.5052501.9671.9451.9561.7921251.2151.1971.2061.04262.50.7310.7200.7260.56231.20.4330.4440.4390.27515.60.3090.3180.3140.1507.80.2430.2510.2470.08300.1650.1630.164 | ||||||
货期：3-5 working days
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