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产品描述：人无花果酶2(FCN2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FCN2含量。试剂盒检测范围为0.25 ng/mL-16 ng/mL，支持科研人员开展免疫调控机制、感染性疾病模型、炎症相关通路及肝脏功能研究，为探索FCN2在宿主防御、免疫稳态及疾病发生发展中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：37 kDa elastin-binding protein ELISA Kit; Collagen/fibrinogen domain containing protein 2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; EBP 37 ELISA Kit; EBP-37 ELISA Kit; EBP37 ELISA Kit; FCN 2 ELISA Kit; Fcn2 ELISA Kit; FCN2_HUMAN ELISA Kit; FCNL ELISA Kit; Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) ELISA Kit; Ficolin B ELISA Kit; Ficolin beta ELISA Kit; Ficolin-2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; Ficolin2 ELISA Kit; Hucolin ELISA Kit; L ficolin ELISA Kit; L-ficolin ELISA Kit; OTTHUMP00000022518 ELISA Kit; P35 ELISA Kit; RP11 263F14.2 ELISA Kit; Serum lectin p35 ELISA Kit
缩写：FCN2
Uniprot No.：Q15485
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.25 ng/mL-16 ng/mL
灵敏度：0.06 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4000 | Average % | 108 | ||||
| Range % | 98-115 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of human FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 101 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 162.0071.9001.9541.844 81.5261.4431.4851.375 41.0971.0091.0530.943 20.6450.6210.6330.523 10.4290.4200.4250.315 0.50.2640.2540.2590.149 0.250.1900.1860.1880.078 00.1100.1090.110 | ||||||
货期：3-5 working days
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