首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血纤蛋白原降解产物(FDP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的FDP含量。FDP即果糖二磷酸，它在细胞能量代谢等方面有重要作用。其背景为机体糖代谢的中间产物。研究机制上，它能调节糖代谢中多种酶活性，增加细胞内高能磷酸键和三磷酸腺苷含量，提高细胞对缺氧、缺血耐受性，利于细胞功能恢复。试剂盒检测范围为1.56 ng/mL-100 ng/mL，常用于心血管疾病、肿瘤转移或炎症反应等机制研究，适用于评估小鼠模型中溶栓治疗效果、血栓形成动态监测或纤维蛋白代谢相关研究，为纤溶系统调控及抗凝药物开发提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：FDP
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of mouse FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 88--95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 1000.1080.1010.105 500.2130.2260.220 250.4270.4110.419 12.50.7850.7530.769 6.251.2241.1471.186 3.121.6791.7291.704 1.561.8941.9291.912 02.3052.4122.359 | ||||||
货期：3-5 working days
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