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产品描述：人血纤肽/纤维蛋白肽A(FPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FPA含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于心血管疾病机制研究、止血与血栓形成相关动物模型分析、抗凝药物开发等科研领域，可为体外凝血功能评估、病理模型构建及药物干预效果评价提供可靠数据支持。实验操作兼容常规酶标仪检测，满足不同实验室对凝血标志物的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGA ELISA Kit; FGB ELISA Kit; FGG ELISA Kit; Fib2 ELISA Kit; FIBA_HUMAN ELISA Kit; Fibrinogen A alpha polypeptide ELISA Kit; Fibrinogen alpha chain ELISA Kit; Fibrinogen B alpha polypeptide ELISA Kit; Fibrinogen beta chain ELISA Kit; Fibrinogen G alpha polypeptide ELISA Kit; Fibrinogen gamma chain ELISA Kit; fibrinogen; B beta polypeptide ELISA Kit; fibrinogen; G gamma polypeptide ELISA Kit; fibrinogen; gamma polypeptide ELISA Kit; Fibrinogen--alpha -polypeptide chain ELISA Kit; Fibrinogen--beta -polypeptide chain ELISA Kit; Fibrinogen--gamma-polypeptide chain ELISA Kit
缩写：FPA
Uniprot No.：P02671
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 107 | ||||
| Range % | 102-115 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 109 | ||||
| Range % | 102-115 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of human FPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-99 | ||||
| EDTA plasma (n=4) | 97 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5542.5752.5652.41751.8501.8891.8701.7222.51.0351.1051.0700.9221.250.6670.6450.6560.5080.6250.4220.4500.4360.2880.3120.3020.2840.2930.1450.1560.2590.2500.2550.10700.1440.1510.148 | ||||||
货期：3-5 working days
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