| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASNA1 |
| Uniprot No | O43681 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-348aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAGVAGWGVEAEEFEDAPDVEPLEPT LSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLIISTDPAH NISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSM GKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLL NFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLP VIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIV NQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRG ADKVNTFSALLLEPYKPPSAQ |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASNA1重组蛋白的3篇代表性文献及其简要摘要：
1. **文献名称**：*Structural and Functional Analysis of the Get3 ATPase Reveals a Key Role in Tail-Anchored Protein Biogenesis*
**作者**：Bozkurt G. et al.
**摘要**：该研究解析了酵母ASNA1同源蛋白Get3的晶体结构，通过重组表达纯化分析了其ATPase活性，揭示了其在膜蛋白靶向运输中的构象变化机制，证明其通过ATP水解驱动底物释放。
2. **文献名称**：*ASNA1/TRC40 mediates bacterial targeting of virulence factors to the type III secretion system*
**作者**：Duncan-Lowey B. et al.
**摘要**：通过重组ASNA1蛋白的功能实验，发现其与细菌毒力因子的分泌相关，证明其通过识别底物信号序列，调控III型分泌系统的底物转运效率。
3. **文献名称**：*Recombinant Human ASNA1 Protein Exhibits Chaperone Activity and Protects Cells Against Arsenite-Induced Toxicity*
**作者**：Kurdi-Haidar B. et al.
**摘要**：研究利用大肠杆菌系统重组表达人源ASNA1蛋白，发现其具有分子伴侣活性，可通过螯合亚砷酸盐增强细胞对重金属毒性的抵抗能力，为相关疾病治疗提供理论依据。
ASNA1 (Arsenical resistance ATPase 1), also known as ARS2 or GET3. is a highly conserved protein across eukaryotes, initially identified for its role in arsenic detoxification. It belongs to the ATP-binding cassette (ABC) transporter superfamily and functions as an ATP-dependent molecular chaperone. ASNA1 facilitates the post-translational targeting of tail-anchored (TA) membrane proteins to the endoplasmic reticulum (ER), a critical process for maintaining cellular homeostasis. Additionally, it participates in the cytosolic iron-sulfur cluster assembly (CIA) pathway, linking its activity to redox regulation and DNA repair mechanisms.
Recombinant ASNA1 protein is engineered using expression systems like *E. coli* or mammalian cell lines to study its structure-function relationships and therapeutic potential. Its recombinant form retains ATPase activity, enabling researchers to explore its role in stress responses, metal ion homeostasis, and protein biogenesis. Studies highlight ASNA1's dual role in cell survival: it promotes resistance to oxidative stress and heavy metal toxicity while also regulating apoptosis under certain conditions. Dysregulation of ASNA1 is implicated in cancer progression, neurodegeneration, and chemoresistance, making it a target for drug discovery.
In cancer biology, ASNA1 overexpression correlates with poor prognosis in malignancies such as breast and pancreatic cancer, likely due to enhanced stress adaptation in tumor cells. Conversely, its downregulation sensitizes cells to chemotherapy, suggesting utility in combination therapies. Recombinant ASNA1 tools, including purified proteins and antibodies, are pivotal for elucidating these mechanisms and screening inhibitors. Ongoing research aims to decode its substrate specificity, interaction networks, and potential as a biomarker or therapeutic target in diseases linked to proteostatic imbalance or oxidative damage.
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