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产品描述：牛碱性成纤维细胞生长因子/肝素结合生长因子2(FGF2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF2含量。FGF2（成纤维细胞生长因子2）是成纤维细胞生长因子家族的重要成员，具有促进细胞生长、分化和血管生成等功能。其研究机制涉及通过结合FGFR受体激活细胞内信号通路，如RAS-MAPK、PI3K-Akt等，参与细胞增殖、分化、迁移和血管生成等生理过程。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于科研领域中对FGF2功能机制的研究，包括但不限于心血管疾病模型、肿瘤微环境分析、创伤愈合过程等研究方向，可为细胞培养上清液、体液样本及组织提取物中的FGF2动态变化提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2) [Cleaved into: Kidney-derived growth factor] ELISA Kit
缩写：FGF2
Uniprot No.：P03969
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 93-102 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 81-89 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 83-95 | ||||||
回收率：
| The recovery of bovine FGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 98-104 | |||||
| EDTA plasma (n=4) | 92 | 89-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4002.0822.2122.1471.9912001.2981.3021.3001.1441000.8260.8850.8560.700500.5270.5640.5460.390250.3410.3440.3430.18712.50.2880.2980.2930.1376.250.2290.2320.2310.07500.1520.1600.156 | |||||||
货期：3-5 working days
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