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产品描述：猪角化细胞生长因子(KGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的FGF7含量。FGF7 即成纤维细胞生长因子 7，对上皮细胞的增殖、分化和迁移起重要作用。其研究机制主要聚焦于与特异性受体结合，激活下游信号通路，影响细胞生理过程。在组织修复、肿瘤发生发展等方面有潜在研究价值。试剂盒检测范围为3.12 pg/mL-200pg/mL，适用于猪模型中与表皮损伤修复、炎症反应或毛发再生相关的分子机制研究。可广泛应用于农业科研中猪皮肤疾病模型构建、药物干预效果评估或生物工程材料促愈活性分析等基础研究场景，为探索KGF在猪生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF7Fibroblast growth factor 7 ELISA kit; FGF-7 ELISA kit; Heparin-binding growth factor 7 ELISA kit; HBGF-7 ELISA kit; Keratinocyte growth factor ELISA kit; KGF ELISA kit
缩写：FGF7
Uniprot No.：Q9N198
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：3.12 pg/mL-200pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 106 | ||||
| Range % | 101-114 | |||||
回收率：
| The recovery of pig KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2001.8151.7311.7731.6321001.1281.1731.1511.010500.7890.8270.8080.667250.5030.5260.5150.37412.50.3120.3090.3110.1706.250.2510.2710.2610.1203.120.1880.1780.1830.04200.1440.1380.141 | ||||||
货期：3-5 working days
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