首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血纤蛋白原γ链(FGG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGG含量。人血纤蛋白原γ链（FGG）是纤维蛋白原分子的重要组成部分，其基因突变会导致遗传性异常纤维蛋白原血症，表现为凝血功能异常，可能引发出血或血栓。研究显示，FGG基因突变会影响纤维蛋白原的结构和功能，进而影响凝血过程。试剂盒检测范围为125 ng/mL-8000 ng/mL，适用于凝血机制研究、心血管疾病模型分析、肝脏功能评估及相关生物标志物探索等科研领域，为体外实验提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGG ELISA Kit; FIBG_HUMAN ELISA Kit; Fibrinogen gamma chain ELISA Kit; Fibrinogen gamma polypeptide ELISA Kit; fibrinogen gamma-b chain ELISA Kit
缩写：FGG
Uniprot No.：P02679
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 ng/mL-8000 ng/mL
灵敏度：31.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FGG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 95-108 | |||||
| 1:400 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:1600 | Average % | 87 | ||||
| Range % | 81-93 | |||||
回收率：
| The recovery of human FGG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 90 | 87-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 80000.0900.0890.090 40000.1180.1240.121 20000.1740.1660.170 10000.2570.2640.261 5000.4720.4460.459 2500.7580.7660.762 1251.0181.1051.062 02.1242.0012.063 | ||||||
货期：3-5 working days
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