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Human Follistatin-related protein 5(FSTL5) ELISA kit

  • 中文名: 人卵泡抑素相关蛋白5(FSTL5)酶联免疫试剂盒
货号: IPD-EL009028HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人卵泡抑素相关蛋白5(FSTL5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FSTL5含量。FSTL5是一种钙离子结合蛋白,可能参与细胞分化和细胞外区域定位。研究发现,FSTL5与青少年痛风新风险基因位点相关,可能通过影响炎症过程参与痛风的发生。此外,FSTL5还与肌肉生长、脂肪棕色化及激素调节等功能有关。试剂盒检测范围为1.25 ng/mL-80 ng/mL,适用于基础医学研究中对FSTL5蛋白表达水平的定量分析,例如探索其在生殖生理调控、组织修复机制或疾病模型中的分子作用。科研人员可通过高效检测生物体液(血清/血浆)中的循环蛋白含量,或分析特定组织匀浆中FSTL5的局部表达特征,为研究其生物学功能及病理相关性提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    FSTL5 ELISA Kit; KIAA1263Follistatin-related protein 5 ELISA Kit; Follistatin-like protein 5 ELISA Kit
  • 缩写:
    FSTL5
  • Uniprot No.:
    Q8N475
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.25 ng/mL-80 ng/mL
  • 灵敏度:
    0.31 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human FSTL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    85-99


    1:2

    Average %

    97


    Range %

    90-104


    1:4

    Average %

    102


    Range %

    95-109


    1:8

    Average %

    92


    Range %

    88-97


  • 回收率:

    The recovery of human FSTL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-101


    EDTA plasma (n=4)

    97

    92-106


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    80

    2.848

    2.694

    2.771

    2.619


    40

    2.465

    2.357

    2.411

    2.259


    20

    1.885

    1.868

    1.877

    1.725


    10

    1.260

    1.229

    1.245

    1.093


    5

    0.720

    0.721

    0.721

    0.569


    2.5

    0.515

    0.533

    0.524

    0.372


    1.25

    0.375

    0.384

    0.380

    0.228


    0

    0.149

    0.155

    0.152




  • 货期:
    3-5 working days

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