首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人铁蛋白轻链(FTL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FTL含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于细胞铁稳态研究、铁代谢相关疾病机制探索或药物干预效果评价等科研场景，满足体外实验中对生物样本FTL含量的精确测定需求，为生命科学基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ferritin L chain ELISA Kit; Ferritin L subunit ELISA Kit; Ferritin light chain ELISA Kit; Ferritin light polypeptide ELISA Kit; ferritin light polypeptide like 3 ELISA Kit; FRIL_HUMAN ELISA Kit; FTL ELISA Kit; LFTD ELISA Kit; NBIA 3 ELISA Kit; NBIA3 ELISA Kit
缩写：FTL
Uniprot No.：P02792
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of human FTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-104 | ||||
| EDTA plasma (n=4) | 93 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected403.0553.1673.1112.987202.3892.2882.3392.215101.4131.4291.4211.29750.8570.8420.8500.7262.50.4620.4830.4730.3491.250.3200.3340.3270.2030.6250.2450.2510.2480.12400.1220.1260.124 | ||||||
货期：3-5 working days
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