首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述:人糖化白蛋白(GA)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的GA含量。人糖化白蛋白(GA)是白蛋白与葡萄糖非酶促糖基化反应的产物,反映近2-3周血糖水平,用于糖尿病诊断、血糖监测和疗效评估。其水平与白蛋白代谢周期相关,对短期血糖控制评估、疗效观察及治疗方案调整具有重要价值。试剂盒检测范围为18.75 pmol/mL-1200 pmol/mL,在糖尿病病理机制研究及血糖代谢相关科研中具有重要价值;适用于糖尿病动物模型研究、降糖药物作用机制探索或代谢性疾病相关基础科研中的GA动态监测需求本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:N/A
缩写:GA
种属:Homo sapiens (Human)
样本类型:serum, plasma
检测范围:18.75 pmol/mL-1200 pmol/mL
灵敏度:4.7 pmol/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:200 | Average % | 94 | ||||
Range % | 90-98 | |||||
1:400 | Average % | 94 | ||||
Range % | 91-98 | |||||
1:800 | Average % | 101 | ||||
Range % | 98-104 | |||||
1:1600 | Average % | 88 | ||||
Range % | 82-96 |
回收率:
The recovery of human GA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 90-98 | ||||
EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pmol/mlOD1OD2Average 12000.0800.0810.081 6000.1170.1180.118 3000.1820.1910.187 1500.2850.2760.281 750.5090.4990.504 37.50.9330.9230.928 18.751.4611.5511.506 02.5392.6682.604 |
货期:3-5 working days
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