首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人谷氨酸脱羧酶65(GAD65)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GAD2含量。GAD2即谷氨酸脱羧酶2，它能催化谷氨酸生成γ-氨基丁酸。其在神经系统中至关重要，参与调节神经系统活动。研究围绕它与神经系统疾病如癫痫、精神分裂症等关联展开，旨在明晰其在发病机制中作用，为开发针对性治疗方法提供依据。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研场景中定量分析不同生物样本的GAD65表达水平，如探究糖尿病动物模型的胰岛功能、神经系统疾病相关蛋白表达动态变化、药物干预对GAD65调控的影响等研究。为神经生物学、免疫学和代谢疾病研究提供稳定可靠的技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：65 kDa glutamic acid decarboxylase ELISA Kit; DCE 2 ELISA Kit; DCE2 ELISA Kit; DCE2_HUMAN ELISA Kit; GAD 2 ELISA Kit; GAD 65 ELISA Kit; GAD-2 ELISA Kit; GAD-65 ELISA Kit; Gad2 ELISA Kit; Glutamate decarboxylase 2 (pancreas) ELISA Kit; Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) ELISA Kit; Glutamate decarboxylase 2 ELISA Kit; Glutamate Decarboxylase 65 ELISA Kit; Glutamate decarboxylase 65 kDa isoform ELISA Kit; Glutamic Acid Decarboxylase 2 ELISA Kit; Glutamic Acid Decarboxylase 65 ELISA Kit; MGC161605 ELISA Kit; MGC161607 ELISA Kit
缩写：GAD2
Uniprot No.：Q05329
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GAD65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of human GAD65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 94-105 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4602.4892.4752.29851.8681.8981.8831.7062.51.3261.3661.3461.1691.250.8530.8790.8660.6890.6250.5330.5740.5540.3770.3120.4040.4360.4200.2430.1560.3190.3450.3320.15500.1780.1760.177 | ||||||
货期：3-5 working days
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