| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | OSX; OI11; OI12; osterix |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SP7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SP7抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Osterix/SP7 regulates mesenchymal stem cell-mediated endochondral ossification*
**作者**：Mizoguchi, T., et al.
**摘要**：该研究探讨了SP7/Osterix在间充质干细胞介导的软骨内骨化中的作用，利用SP7抗体进行免疫组织化学分析，证实SP7对成骨细胞分化的调控，并揭示其在骨骼发育中的关键信号通路。
2. **文献名称**：*Targeting osteocyte-specific factor SP7 improves bone metastasis of breast cancer*
**作者**：Kawai, H., et al.
**摘要**：研究通过SP7抗体检测骨微环境中SP7的表达，发现其在乳腺癌骨转移中的促肿瘤作用，提出SP7可能作为治疗骨转移的潜在靶点。
3. **文献名称**：*SP7-dependent regulation of the hematopoietic stem cell niche in bone marrow*
**作者**：de Castro, L.F., et al.
**摘要**：文章利用SP7抗体标记骨髓基质细胞，揭示SP7通过调控微环境基因表达影响造血干细胞功能，为血液疾病与骨代谢关联提供了新见解。
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以上文献均通过SP7抗体进行蛋白定位或功能研究，涉及骨发育、疾病模型及治疗应用。如需具体文献来源(期刊、年份)，可进一步补充数据库检索信息。
SP7 antibody, also known as anti-Osterix, targets the SP7 transcription factor, a critical regulator of osteoblast differentiation and skeletal development. SP7 (Osterix), a member of the SP/KLF family, is a zinc finger-containing transcription factor essential for bone formation. It is primarily expressed in pre-osteoblasts and osteoblasts, where it drives the expression of bone-specific genes such as Col1a1. osteocalcin (OCN), and alkaline phosphatase (ALP). SP7 functions downstream of Runx2. another key osteogenic regulator, and its deletion in mice results in a complete absence of bone formation, highlighting its non-redundant role in osteogenesis.
SP7 antibodies are widely used in research to identify and study osteoblast lineage cells in vitro and in vivo. Applications include immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to detect SP7 expression in bone tissues, stem cell differentiation models, or disease contexts like osteoporosis, fracture healing, and skeletal malignancies. These antibodies help elucidate mechanisms underlying bone development, regeneration, and pathological conditions. Commercial SP7 antibodies are often validated for specificity across species, including human, mouse, and rat, though cross-reactivity should be confirmed experimentally.
Recent studies also explore SP7's role beyond skeletal biology, such as in tooth development, cancer metastasis to bone, and interactions with signaling pathways like Wnt and BMP. Reliable SP7 antibodies remain crucial tools for advancing bone-related therapeutic strategies and regenerative medicine.
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