首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胰高血糖素(GC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GCG含量。GCG（胰高血糖素）是一种重要靶点。它在血糖调节等生理过程中起关键作用。研究机制上，GCG通过与受体结合，激活细胞内信号通路，影响糖原分解、糖异生等代谢途径。围绕它开展的研究有助于深入了解代谢调控机制及相关疾病防治。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于能量代谢调控、糖代谢机制及糖尿病相关动物模型的科学研究；能够满足体外实验中GC动态监测、组织分布分析及干预效果评价等科研需求，为代谢性疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GcgGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA kit; GRPP); Oxyntomodulin ELISA kit; OXM ELISA kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA kit; GLP-2)] ELISA kit
缩写：GCG
Uniprot No.：P55095
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 93-110 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 88-100 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 82-104 | |||||
回收率：
| The recovery of mouse GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 85-99 | ||||
| EDTA plasma (n=4) | 95 | 86-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5642.5922.5782.489501.4391.4881.4641.375250.8050.7920.7990.71012.50.4210.4320.4270.3386.250.2140.2230.2190.1303.120.1460.1500.1480.0591.560.1020.1050.1040.01500.0880.0900.089 | ||||||
货期：3-5 working days
×
