首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：大鼠粒细胞集落刺激因子(G-CSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的G-CSF含量。G-CSF即粒细胞集落刺激因子，在造血调控中至关重要。它能特异性地作用于造血干细胞，促进粒细胞前体细胞增殖、分化，增强成熟粒细胞功能。临床常用于放化疗后骨髓抑制等，提升粒细胞数量，降低感染风险，研究聚焦其信号通路及新应用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，在基础研究中可用于探索免疫系统调控机制、炎症相关疾病模型中的G-CSF动态变化，或评估药物/化合物对中性粒细胞生成的影响。例如在感染性疾病模型、骨髓抑制恢复研究或组织损伤修复实验中，可通过检测G-CSF表达水平揭示其生物学功能。试剂盒操作流程标准化，配备预包被96孔板及优化反应体系，适用于实验室常规批量样本检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
缩写：G-CSF
Uniprot No.：P48750
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-97 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-96 | ||||
| EDTA plasma (n=4) | 94 | 92-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1332.1182.1262.00710001.8371.7761.8071.6885001.2941.2821.2881.1692500.8890.8780.8840.7651250.5880.6070.5980.47962.50.3610.3490.3550.23631.250.2220.2160.2190.10000.1180.1200.119 | ||||||
货期：3-5 working days
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