首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胶质细胞系来源的神经营养因子(GDNF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GDNF含量。胶质细胞源性神经营养因子（GDNF）属转化生长因子 -β超家族，是重要神经营养因子。它能促进多巴胺能、运动、感觉等神经元存活与生长。研究机制主要是通过结合受体激活下游信号通路，在神经系统发育、损伤修复等方面有潜在治疗价值。试剂盒检测范围为78 pg/mL-5000 pg/mL，广泛应用于神经退行性疾病机制研究、药物干预效果评价以及体外细胞模型中GDNF动态变化的监测，特别适用于探究GDNF在神经保护、再生修复等生物学过程中的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Astrocyte derived trophic factor ELISA Kit; Astrocyte derived trophic factor 1 ELISA Kit; Astrocyte-derived trophic factor ELISA Kit; ATF 1 ELISA Kit; ATF 2 ELISA Kit; Atf ELISA Kit; ATF1 ELISA Kit; ATF2 ELISA Kit; gdnf ELISA Kit; GDNF_HUMAN ELISA Kit; Glial cell derived neurotrophic factor ELISA Kit; Glial Cell Line Derived Neurotrophic Factor ELISA Kit; Glial cell line-derived neurotrophic factor ELISA Kit; Glial derived neurotrophic factor ELISA Kit; HFB1 GDNF ELISA Kit; hGDNF ELISA Kit; HSCR3 ELISA Kit
缩写：GDNF
Uniprot No.：P39905
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of human GDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 97 | 93-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 50002.0661.9522.0091.848 25001.8561.7761.8161.655 12501.5421.5231.5331.372 6251.1221.1371.1300.969 312.50.7090.6840.6970.536 156.250.4780.4590.4690.308 780.2960.3040.3000.139 00.1630.1590.161 | ||||||
货期：3-5 working days
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