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产品描述：人神经胶质纤维酸性蛋白(GFAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GFAP含量。GFAP（胶质纤维酸性蛋白）是星形胶质细胞的一种中间丝蛋白。其背景在于它是反映星形胶质细胞状态的重要标志物。研究机制方面，常通过检测其表达水平变化，来探究中枢神经系统损伤、神经退行性疾病等病理过程中星形胶质细胞的活化与反应情况。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于神经退行性疾病机制研究、脑损伤动物模型评估、体外细胞实验中的胶质细胞活化分析等科研场景，可为中枢神经系统疾病相关生物标志物探索、药物干预效果评价及病理模型构建提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GFAP ELISA Kit; GFAP Epsilon ELISA Kit; GFAP_HUMAN ELISA Kit; GFAPdelta ELISA Kit; GFAPepsilon ELISA Kit; Glial fibrillary acidic protein ELISA Kit; Intermediate filament protein ELISA Kit
缩写：GFAP
Uniprot No.：P14136
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 98-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 89-96 | |||||
回收率：
| The recovery of human GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-104 | ||||
| EDTA plasma (n=4) | 93 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.4372.5782.5082.412202.2412.2562.2492.153101.7781.7901.7841.68851.2051.2241.2151.1192.50.7120.7380.7250.6291.250.5040.5290.5170.4210.6250.2480.2670.2580.16200.0950.0970.096 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GFAP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GFAP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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