首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠GDNF家族α样受体(GFRAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GFRAL含量。GFRAL是一个重要靶点。它与机体能量代谢等生理过程密切相关。研究机制上，其能参与特定信号通路，可能成为调节能量平衡的关键环节。目前围绕它正开展众多科研工作，有望为肥胖症等代谢疾病治疗带来新策略。试剂盒检测范围为25 pg/mL-1600 pg/mL，本试剂盒通过高特异性配对抗体实现目标蛋白的精准捕获与信号放大，适用于基础科研领域探索GFRAL在代谢性疾病动物模型中的动态变化、评估特定组织或细胞中受体表达差异，以及筛选调控GFRAL通路的药物或生物活性分子。产品严格验证了线性范围与灵敏度指标，可满足体外实验中低丰度蛋白的稳定检测需求，为代谢机制研究、神经内分泌调控及疾病模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
缩写：GFRAL
Uniprot No.：Q6SJE0
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-98 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-100 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16001.8591.9521.9061.7668001.2581.2601.2591.1194000.7200.7340.7270.5872000.4660.4890.4780.3381000.3150.3280.3220.182500.2290.2350.2320.092250.1720.1760.1740.03400.1380.1410.140 | |||||||
货期：3-5 working days
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