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产品描述：鸡生长激素(GH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GH含量。GH即生长激素，在人体生长发育、代谢等方面扮演关键角色。其研究机制主要聚焦于通过与生长激素受体结合，激活相关信号通路，调节细胞增殖、分化和代谢等过程。目前围绕GH在生长发育异常、代谢疾病等领域的研究不断深入。试剂盒检测范围为625 pg/mL-10000 pg/mL，探究家禽生长发育调控机制、评估营养干预对激素分泌的影响、建立疾病相关代谢模型、农业科研领域研究家禽生理调控、品种选育及饲养管理优化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：GH
Uniprot No.：P08998
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：625 pg/mL-10000 pg/mL
灵敏度：625 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of chicken GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 88-96 | ||||
| EDTA plasma (n=4) | 94 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 100000.1540.1450.150 50000.2610.2440.253 25000.5380.5340.536 12501.1131.1391.126 6252.3562.2782.317 | ||||||
货期：3-5 working days
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