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Mouse Glycated hemoglobin A1c,GHbA1c ELISA Kit

  • 中文名: 小鼠糖化血红蛋白A1c(GHbA1c)ELISA Kit
货号: IPD-E08141m
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠糖化血红蛋白A1c(GHbA1c)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测lysatesforRBC样本中的GHbA1c含量。GHbA1c即糖化血红蛋白,反映过去 2 至 3 个月的平均血糖水平。它是评估糖尿病长期血糖控制的重要指标。研究机制上,其由血红蛋白与葡萄糖非酶促结合而成,结合量与血糖浓度正相关,可更全面反映血糖波动情况。试剂盒检测范围为62.5 ng/ml-4000 ng/ml.,适用于科研实验中红细胞裂解液样本的定量分析;常用于糖尿病相关机制研究及代谢性疾病模型的评估;适用于体外实验中对红细胞样本的糖化程度分析,可应用于糖尿病动物模型研究、降糖药物作用机制探索或慢性高血糖对红细胞功能影响的科研项目中本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    GHbA1c
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    lysates for RBC
  • 检测范围:
    62.5 ng/ml-4000 ng/ml.
  • 灵敏度:
    15.6ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GHbA1c in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Lysate for RBC(n=4)


    1:200

    Average %

    84


    Range %

    80-96


    1:400

    Average %

    90


    Range %

    83-99


    1:800

    Average %

    87


    Range %

    82-100


    1:1600

    Average %

    85


    Range %

    82-98


  • 回收率:

    The recovery of mouse GHbA1c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Lysate for RBC (n=5)

    85

    82-95


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average




    4000

    0.176

    0.184

    0.180




    2000

    0.259

    0.267

    0.263




    1000

    0.363

    0.378

    0.370




    500

    0.520

    0.529

    0.525




    250

    0.794

    0.821

    0.807




    125

    1.174

    1.233

    1.204




    62.5

    1.724

    1.684

    1.704




    0

    2.608

    2.625

    2.616





  • 货期:
    3-5 working days

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