Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

大包装询价

货号: IPD-E08118m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠胰高血糖素样肽1(GLP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的GLP-1含量。GLP-1（胰高血糖素样肽-1）是一种由肠道L细胞分泌的激素，能促进胰岛β细胞分泌胰岛素，抑制胰高血糖素的分泌，并作用于大脑食欲调节中枢，抑制食欲，从而降低血糖和体重。其研究机制涉及调节血糖、减重和心血管保护等多重作用。试剂盒检测范围为125 pg/ml-8000 pg/ml.，适用于科研领域中对GLP-1表达水平的定量分析，如糖尿病发病机制研究、代谢调控药物筛选、肠道激素功能探索等方向，可为体外实验提供稳定可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
N/A
缩写：
GLP-1
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
125 pg/ml-8000 pg/ml.
灵敏度：
31.25 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	104
	Range %	98-110
1:2	Average %	95
	Range %	91-105
1:4	Average %	101
	Range %	94-108
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
8000	2.397	2.334	2.365	2.173
4000	1.701	1.667	1.684	1.492
2000	1.162	1.098	1.130	0.938
1000	0.788	0.819	0.803	0.611
500	0.572	0.551	0.562	0.370
250	0.413	0.423	0.418	0.226
125	0.302	0.294	0.298	0.106
0	0.189	0.195	0.192

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse GLP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GLP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.