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产品描述：小鼠谷胱甘肽相关蛋白5，线粒体(GLRX5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GLRX5含量。GLRX5是一个有研究价值的靶点。它在人体内参与铁硫簇的生物合成等重要生理过程。其异常可能与多种疾病相关，如血液系统疾病等。目前研究聚焦于其在疾病发生发展机制中的作用，以探寻基于此靶点的潜在治疗策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探究GLRX5在氧化应激、线粒体功能障碍或铁代谢异常等病理生理模型中的表达变化，例如通过检测肝、脑等组织或培养细胞中的蛋白水平，揭示其在神经退行性疾病、代谢综合征或贫血相关研究中的作用机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Glrx5Glutaredoxin-related protein 5 ELISA Kit; mitochondrial ELISA Kit; Monothiol glutaredoxin-5 ELISA Kit
缩写：GLRX5
Uniprot No.：Q80Y14
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLRX5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 90-96 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
回收率：
| The recovery of mouse GLRX5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-102 | |||||
| EDTA plasma (n=4) | 106 | 102-113 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.6392.5242.5822.431 10002.1722.0682.1201.969 5001.5931.5231.5581.407 2500.9250.8920.9090.758 1250.5870.5710.5790.428 62.50.3680.3460.3570.206 31.250.2560.2470.2520.101 00.1490.1530.151 | |||||||
货期：3-5 working days
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