首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠谷氨酸[NMDA]受体epsilon-2亚基(GRIN2B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GRIN2B含量。GRIN2B是重要的药物靶点。它参与调控神经信号传导等过程，在神经系统功能维持中发挥关键作用。目前研究聚焦其在神经系统疾病如阿尔茨海默病、癫痫中的机制，旨在明确其作用通路，为开发针对性治疗药物提供理论支撑。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒可广泛应用于神经科学领域的基础研究，包括阿尔茨海默病模型、脑损伤机制探索、突触功能调控实验以及药物对NMDA受体通路影响的评估，为研究GRIN2B在神经系统生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Grin2bGlutamate receptor ionotropic ELISA Kit; NMDA 2B ELISA Kit; GluN2B ELISA Kit; Glutamate [NMDA] receptor subunit epsilon-2 ELISA Kit; N-methyl D-aspartate receptor subtype 2B ELISA Kit; NMDAR2B ELISA Kit; NR2B ELISA Kit
缩写：GRIN2B
Uniprot No.：Q01097
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GRIN2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 90-96 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:4 | Average % | 105 | |||||
| Range % | 100-114 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of mouse GRIN2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 92-100 | |||||
| EDTA plasma (n=4) | 87 | 83-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.6832.5792.6312.48510002.1592.0522.1061.9605001.4941.5211.5081.3622500.9160.8690.8930.7471250.5870.6070.5970.45162.50.3730.3830.3780.23231.250.2650.2550.2600.11400.1490.1420.146 | |||||||
货期：3-5 working days
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