首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝溶胶蛋白(Gelsolin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的GSN含量。GSN即凝溶胶蛋白，是一种多功能肌动蛋白结合蛋白。其背景上，在细胞内分布广泛且具多种功能。研究机制方面，它参与细胞运动、凋亡等过程，还与多种疾病相关，如肿瘤、神经系统疾病等，对其深入研究有助于探索疾病发病机制与治疗靶点。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于体外实验中定量评估Gelsolin在细胞功能研究、炎症模型构建、神经退行性疾病或肿瘤微环境等领域的表达变化；在细胞迁移、免疫调控及病理机制研究中具有重要价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gsn ELISA kit; GsbGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
缩写：GSN
Uniprot No.：P13020
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gelsolin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 102 | |||||
| Range % | 98-107 | ||||||
| 1:200 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:400 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:800 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of mouse Gelsolin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected10002.7412.5782.6602.5565001.9031.8271.8651.7612501.1951.2101.2031.0991250.6500.6850.6680.56462.50.3780.3620.3700.26631.20.2620.2490.2560.15215.60.1840.1910.1880.08400.1010.1060.104 | |||||||
货期：3-5 working days
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