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产品描述：人甲肝病毒细胞受体2(HAVCR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HAVCR2含量。HAVCR2，也称为TIM-3，是一种在T细胞上表达的跨膜蛋白，属于TIM家族。它通过结合多种配体如CD244和磷脂酰丝氨酸（PS）来调节免疫应答，主要作为抑制性受体参与T细胞活性调节。HAVCR2在自身免疫性疾病、感染性疾病、肿瘤免疫监视和免疫逃逸等免疫反应中发挥重要作用，是适应性免疫中的抑制分子，且在某些肿瘤中表达水平的变化与疾病进展相关。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于血清、血浆及组织匀浆样本的科研分析；适用于探索感染性疾病中宿主免疫应答机制、肿瘤微环境免疫抑制研究，以及免疫相关疾病模型的生物标志物分析，为免疫调控机制研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD366 ELISA Kit; FLJ14428 ELISA Kit; HAVcr-2 ELISA Kit; Havcr2 ELISA Kit; HAVR2_HUMAN ELISA Kit; Hepatitis A virus cellular receptor 2 ELISA Kit; Kidney injury molecule 3 ELISA Kit; KIM 3 ELISA Kit; KIM3 ELISA Kit; T cell immunoglobulin and mucin domain containing 3 ELISA Kit; T cell immunoglobulin mucin 3 ELISA Kit; T-cell immunoglobulin and mucin domain-containing protein 3 ELISA Kit; T-cell immunoglobulin mucin family member 3 ELISA Kit; T-cell immunoglobulin mucin receptor 3 ELISA Kit; T-cell membrane protein 3 ELISA Kit; Tim 3 ELISA Kit; TIM-3 ELISA Kit; TIM3 ELISA Kit; TIMD-3 ELISA Kit; TIMD3 ELISA Kit
缩写：HAVCR2
Uniprot No.：Q8TDQ0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HAVCR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-108 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 86-104 | |||||
回收率：
| The recovery of human HAVCR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-106 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.3012.1952.2482.14515001.5271.4471.4871.3847500.9440.9260.9350.8323750.5990.6110.6050.502187.50.4120.4180.4150.312940.2600.2600.2600.157470.1810.1770.1790.07600.1030.1020.103 | ||||||
货期：3-5 working days
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