首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血红蛋白α亚基(HB-α)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、RBC裂解物样本中的HBA1含量。HBA1编码成人血红蛋白的α亚基，在氧气运输等生理过程中至关重要。其异常与多种血红蛋白病相关。研究主要聚焦基因变异对蛋白结构和功能的影响，以揭示疾病发病机制，为开发针对性诊断方法和治疗策略提供依据。试剂盒检测范围为0.170 ng/mL-700 ng/mL，适用于体外培养红细胞研究、血红蛋白合成调控实验、溶血性贫血动物模型评估等科研场景；满足基础研究与转化医学中对HB-α蛋白定量分析的需求，为探索血液相关疾病的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha 1 globin ELISA Kit; Alpha globin ELISA Kit; Alpha one globin ELISA Kit; Alpha-globin ELISA Kit; HBA_HUMAN ELISA Kit; HBA1 ELISA Kit; HBA2 ELISA Kit; Hemoglobin alpha 1 ELISA Kit; Hemoglobin alpha 1 chain ELISA Kit; Hemoglobin alpha 1 globin chain ELISA Kit; Hemoglobin alpha 2 ELISA Kit; Hemoglobin alpha chain ELISA Kit; Hemoglobin subunit alpha ELISA Kit; MGC126895 ELISA Kit; MGC126897 ELISA Kit
缩写：HBA2
Uniprot No.：P69905
种属：Homo sapiens (Human)
样本类型：serum, plasma, lysate for RBC
检测范围：0.170 ng/mL-700 ng/mL
灵敏度：0.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HB-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 93 | |||||
| Range % | 84-103 | ||||||
| 1:200 | Average % | 91 | |||||
| Range % | 85-99 | ||||||
| 1:400 | Average % | 95 | |||||
| Range % | 85-103 | ||||||
| 1:800 | Average % | 91 | |||||
| Range % | 83-98 | ||||||
回收率：
| The recovery of human HB-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 88-101 | |||||
| EDTA plasma (n=4) | 95 | 87-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average7000.2480.2520.2501750.4410.4350.438440.5280.5140.521110.7120.7230.7182.70.8670.8610.8640.71.1191.1071.1130.171.7381.7161.72701.9351.9221.929 | |||||||
货期：3-5 working days
×
