首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人高糖基化人绒毛膜促性腺激素(hCG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液样本中的hCG含量。hCG即人绒毛膜促性腺激素，是妊娠时胎盘滋养层细胞分泌的糖蛋白。在医学研究中，其可用于妊娠诊断。研究机制上，它能促进黄体发育，维持妊娠状态，还和肿瘤发生发展相关，在肿瘤诊断和治疗靶点研究受关注。试剂盒检测范围为0.67 mIU/mL-20 mIU/mL，该产品适用于生殖生物学研究，如妊娠相关分子机制探索、滋养层细胞功能分析，也可用于肿瘤生物学领域中对hCG异常表达的机制研究，或体外细胞模型中激素分泌水平的动态监测。通过标准化的实验流程，为科研工作者提供稳定可靠的数据支持，满足生殖医学、细胞生物学及疾病模型构建等领域的高灵敏度检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：hCG
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates
检测范围：0.67 mIU/mL-20 mIU/mL
灵敏度：0.167 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human hCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-101 | |||||
回收率：
| The recovery of human hCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 91-103 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 202.5422.5392.5412.493 8.31.2821.2761.2791.231 3.30.6420.6340.6380.590 1.30.2880.2850.2870.239 0.670.1560.1490.1530.105 00.0490.0470.048 | ||||||
货期：3-5 working days
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