首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠低氧诱导因子1α(HIF-1α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的HIF1A含量。HIF1A是细胞对低氧环境适应的关键调节因子。其背景在于在肿瘤、心血管疾病等病理过程中异常活跃。研究机制上，它在低氧条件下稳定表达，调控下游基因转录，参与血管生成、糖代谢等过程，是当下疾病治疗靶点研究热点。试剂盒检测范围为0.625 pg/mL-40 pg/mL，适用于基础科研中低氧相关疾病模型的机制研究，如肿瘤微环境分析、心血管疾病病理探索或代谢调控实验，可为体外培养细胞上清、动物血清/血浆样本中的HIF-1α动态变化提供可靠检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hif1aHypoxia-inducible factor 1-alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF1-alpha ELISA Kit; ARNT-interacting protein ELISA Kit
缩写：HIF1A
Uniprot No.：Q61221
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.625 pg/mL-40 pg/mL
灵敏度：0.156 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 100 | ||||
| Range % | 95-107 | |||||
| 1:200 | Average % | 89 | ||||
| Range % | 83-98 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of mouse HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected402.2532.2332.2432.186201.3461.2751.3111.254100.7920.7520.7720.71550.4680.4910.4800.4232.50.2630.2640.2640.2071.250.2220.2400.2310.1740.6250.1180.1220.1200.06300.0550.0580.057 | ||||||
货期：3-5 working days
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