关键信息
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基因名
MAPKAPK3
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简介
The MAPKAPK3 protein is a stress-activated kinase involved in multiple cellular processes, including cytokine production and endocytosis. It is phosphorylated by p38-alpha/MAPK14 during stress, targets specific substrates, and has similarities to MAPKAPK2 but is less active. MAPKAPK3 Protein, Human (Active, sf9, GST) is the recombinant human-derived MAPKAPK3, expressed by Sf9 insect cells, with GST labeled tag.
- 应用
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种属
Human
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表达系统
Baculovirus
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标签
GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q16644
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表达区间
D2-Q382
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MAPKAPK3 (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3) is a serine-threonine kinase that plays a critical role in various cellular processes, including cell proliferation, differentiation, and response to stress. It acts downstream of the p38 MAPK pathway, which is activated by various stimuli such as cytokines, growth factors, and environmental stresses. The understanding of MAPKAPK3's functions has expanded in recent years, revealing its involvement in several pathological conditions, including inflammation, neurodegenerative diseases, and cancer. In particular, MAPKAPK3 regulates the stability of mRNA and the translation of stress response proteins, which are vital for cellular adaptation to adverse conditions. Given its prominent role in mediating cellular responses, MAPKAPK3 has emerged as a potential therapeutic target. Recent studies have focused on the development and characterization of recombinant MAPKAPK3 proteins to elucidate their biochemical properties and interactions with substrates or inhibitors. These investigations aim to provide deeper insights into its regulatory mechanisms and biological functions, paving the way for novel interventions in diseases associated with dysregulated MAPKAPK3 activity. Understanding the structure-function relationship of MAPKAPK3 through recombinant proteins can lead to targeted drug design and the development of therapeutic strategies that modulate its activity for clinical applications.
