关键信息
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基因名
S100A8-S100A9
- 应用
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种属
Mouse
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P27005 (P2-E89) (S100A8) & P31725 (A2-K113)
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表达区间
P27005 (P2-E89) (S100A8)&P31725 (A2-K113) (S100A9)
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氨基酸序列
S100A8: PSELEKALSNLIDVYHNYSNIQGNHHALYKNDFKKMVTTECPQFVQNINIENLFRELDINSDNAINFEEFLAMVIKVGVASHKDSHKE S100A9: ANKAPSQMERSITTIIDTFHQYSRKEGHPDTLSKKEFRQMVEAQLATFMKKEKRNEALINDIMEDLDTNQDNQLSFEECMMLMAKLIFACHEKLHENNPRGHGHSHGKGCGK
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分子量
11&12.9 kDa
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内毒素
<1 EU/μg, determined by LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
S100A8 and S100A9 are calcium-binding proteins belonging to the S100 family, known for their roles in various cellular processes including inflammation, cell differentiation, and apoptosis. These proteins often form a heterodimer, S100A8-S100A9, which has garnered significant attention in recent years due to its involvement in the pathogenesis of several inflammatory diseases and cancer. Elevated levels of this complex have been observed in conditions such as rheumatoid arthritis, inflammatory bowel disease, and tumors, highlighting its potential as a biomarker for disease activity and severity. The S100A8-S100A9 complex functions as a damage-associated molecular pattern (DAMP), actively participating in the recruitment and activation of immune cells, which can exacerbate inflammatory responses. Due to its critical role in inflammation and immunity, research has focused on the therapeutic potential of targeting S100A8-S100A9 in various diseases. Recombinant proteins of S100A8 and S100A9 have been developed to facilitate in vivo studies aimed at understanding their biological functions and therapeutic implications. These studies are essential in elucidating the mechanisms by which S100A8-S100A9 contributes to disease processes, thereby informing potential strategies for intervention and treatment in inflammatory and oncological contexts. Moreover, understanding the structural properties and interactions of S100A8-S100A9 may pave the way for the development of novel therapeutic agents that can modulate their activity, providing new avenues for medical research.
