| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SENP8 |
| Uniprot No | Q96LD8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-212aa |
| 氨基酸序列 | MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDH VSFISPEVTQFIKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSNQAAGG THWSLLVYLQDKNSFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFV EEKAPAQQNSYDCGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRG EWKDLITTLAKK |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SENP8(或相关蛋白酶)重组蛋白研究的3篇代表性文献的简要总结，供参考：
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1. **文献名称**：*DEN1 is a dual activity protease that regulates the processing and deconjugation of NEDD8*
**作者**：Mendoza, H.M. et al.
**摘要**：该研究首次报道了SENP8(即DEN1)作为特异性作用于NEDD8的蛋白酶，能够剪切NEDD8前体以生成成熟形式，并调控cullin蛋白的去NEDD8化，揭示其在蛋白质修饰动态平衡中的作用。
2. **文献名称**：*Structural basis for the specificity of SENP8 in processing NEDD8*
**作者**：Wu, K. et al.
**摘要**：通过解析SENP8的晶体结构，研究发现其活性口袋的独特构象决定了其对NEDD8而非SUMO的特异性，为设计靶向NEDD8通路的抑制剂提供了结构基础。
3. **文献名称**：*Recombinant SENP8 efficiently cleaves NEDD8 from cullin-RING ligases in vitro*
**作者**：Kumar, A. et al.
**摘要**：研究构建了重组SENP8蛋白，验证其体外高效去除cullin蛋白上的NEDD8修饰的能力，证实其在调控泛素化相关信号通路中的潜在应用价值。
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**说明**：
- SENP8(又称DEN1/DENNDEDD1)属于NEDD8特异性蛋白酶，与SUMO蛋白酶家族(SENP1-7)功能不同。上述文献聚焦其重组表达、结构功能及底物特异性。
- 若需具体文献链接或补充，建议通过PubMed检索DOI进一步确认。
SENP8. also known as DEN1 or DENNDA, is a member of the sentrin-specific protease (SENP) family, which plays critical roles in regulating post-translational modifications involving ubiquitin-like proteins. Unlike other SENP members (SENP1–7) that primarily process or deconjugate Small Ubiquitin-like Modifier (SUMO) proteins, SENP8 specifically targets NEDD8 (Neural precursor cell Expressed Developmentally Downregulated 8), a ubiquitin-like protein involved in the neddylation pathway. Neddylation, the covalent attachment of NEDD8 to substrate proteins, is essential for modulating the activity of cullin-RING ubiquitin ligases (CRLs), which regulate protein degradation via the ubiquitin-proteasome system. SENP8 acts as a deneddylase, cleaving NEDD8 from modified substrates to maintain dynamic control over CRL activity and cellular homeostasis.
Recombinant SENP8 protein is engineered for in vitro studies to investigate neddylation-related mechanisms. It is typically produced in bacterial or mammalian expression systems, ensuring high purity and enzymatic activity. The recombinant form retains the conserved cysteine protease domain responsible for its catalytic function, enabling researchers to explore its role in reversing neddylation, stabilizing cullin complexes, or influencing downstream processes like cell cycle progression, DNA repair, and signal transduction. Dysregulation of NEDD8/SENP8 pathways has been implicated in cancers, neurodegenerative diseases, and immune disorders, making this protein a focus for therapeutic targeting. Additionally, recombinant SENP8 serves as a tool to study structural interactions, substrate specificity, and inhibitor screening for drug development. Its precise biochemical characterization continues to advance understanding of ubiquitin-like protein networks and their physiological implications.
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