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产品描述：犬高迁移率族蛋白B1(HMGB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HMGB1含量。HMGB1是一种高度保守的DNA结合蛋白，广泛存在于真核细胞内。它参与多种生理过程如DNA修复等。在研究机制上，HMGB1可在细胞坏死时被动释放或经免疫细胞激活后主动分泌，与多种受体相互作用，介导炎症反应等病理过程，是疾病治疗研究的潜在靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域对犬类炎症性疾病模型、免疫调控机制或药物干预效果的研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HMGB1 ELISA Kit; High mobility group protein B1 ELISA Kit; High mobility group protein 1 ELISA Kit; HMG-1 ELISA Kit
缩写：HMGB1
Uniprot No.：Q6YKA4
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog HMGB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 99-107 | ||||||
回收率：
| The recovery of dog HMGB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 80-90 | |||||
| EDTA plasma (n=4) | 97 | 92-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.6732.6312.6522.526101.5491.5181.5341.40850.9040.9520.9280.8022.50.6120.6290.6210.4951.250.3960.4070.4020.2760.6250.2810.2690.2750.1490.3120.1570.1650.1610.03500.1280.1240.126 | |||||||
货期：3-5 working days
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