首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠富组氨酸糖蛋白(HRG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HRG含量。小鼠富组氨酸糖蛋白（HRG）是一种位于血浆和血小板中的糖蛋白，其生理功能尚不明确，但已知可结合血红素、染料和二价金属离子，并可能具有抗菌活性。HRG在凝血过程中可能通过抑制凝血因子FXIIa的活性来调节血栓形成，其功能受蛋白二硫键异构酶PDI的调控。试剂盒检测范围为6.25 ng/mL-400 ng/mL，其在小鼠模型中的表达水平变化常被用于研究感染性疾病、肿瘤微环境调控及代谢紊乱等科研领域；为探索HRG在凝血功能异常、炎症反应或免疫相关疾病模型中的分子机制提供可靠工具，特别适用于基础医学研究、药效评价及分子病理学等科研场景，助力研究者深入解析HRG在疾病发生发展中的生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HrgHistidine-rich glycoprotein ELISA Kit; Histidine-proline-rich glycoprotein ELISA Kit; HPRG ELISA Kit
缩写：HRG
Uniprot No.：Q9ESB3
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HRG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 87 | ||||
| Range % | 81-96 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 83-97 | |||||
| 1:4000 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of mouse HRG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-100 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average4000.1440.1460.1452000.2260.2320.2291000.3710.3770.374500.5390.5640.552250.9650.9920.97912.51.7041.5631.6346.252.3412.3592.35002.7612.7502.756 | ||||||
货期：3-5 working days
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