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产品描述：猪热休克蛋白70(HSP-70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSP70含量。HSP70 是重要的分子伴侣，在细胞应激反应中起关键作用，可保护细胞免受损害。研究表明，它与肿瘤发生、发展及神经退行性疾病相关。其作用机制主要是协助蛋白质折叠、转运和降解，维持细胞内蛋白稳态，如今是药物研发的热门靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于畜牧科研领域，可用于探究环境胁迫对猪生理状态的影响、疾病模型中的应激响应机制，或作为评估猪群健康状态的分子标志物，为动物营养调控、抗逆育种及病理学研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HSPA1A ELISA Kit; HSPA1Heat shock 70 kDa protein 1A ELISA Kit; Heat shock 70 kDa protein 1 ELISA Kit; HSP70.1 ELISA Kit
缩写：HSP-70
Uniprot No.：P34930
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-107 | |||||
回收率：
| The recovery of pig HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-99 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 401.9521.9311.9421.807 201.5701.6131.5921.457 101.2351.2681.2521.117 50.9110.8970.9040.769 2.50.6720.6560.6640.529 1.250.4090.3950.4020.267 0.6250.2650.2510.2580.123 00.1380.1310.135 | ||||||
货期：3-5 working days
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