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产品描述:人热休克蛋白HSP90β(HSP90AB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HSP90AB1含量。HSP90AB1,又称HSP90 beta,是HSP90家族成员之一,参与细胞调节,与多种细胞激酶、转录因子等形成复合物。其在多种癌症中表达上调,具有信号转导、蛋白质折叠和降解等功能。研究机制涉及HSP90AB1与病毒N、NS7和NSP10蛋白互作,通过蛋白酶体途径调节N蛋白稳定性,影响病毒增殖。试剂盒检测范围为1.56 ng/mL-100 ng/mL,本试剂盒适用于科研领域中对HSP90β表达调控机制的研究,例如在细胞应激模型、肿瘤微环境分析或疾病相关信号通路探索中定量检测目标蛋白的动态变化。为体外实验提供可靠的定量数据支持,满足基础研究中对HSP90β功能验证及分子互作分析的实验需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:90 kda heat shock protein beta HSP90 beta ELISA Kit; D6S182 ELISA Kit; FLJ26984 ELISA Kit; Heat shock 84 kDa ELISA Kit; Heat shock 90kD protein 1; beta ELISA Kit; Heat shock 90kDa protein 1 beta ELISA Kit; Heat shock protein 90 alpha family class B member 1 ELISA Kit; Heat shock protein 90 kDa ELISA Kit; Heat shock protein 90kDa alpha (cytosolic) class B member 1 ELISA Kit; Heat shock protein 90kDa alpha family class B member 1 ELISA Kit; Heat shock protein beta ELISA Kit; Heat shock protein HSP 90 beta ELISA Kit; Heat shock protein HSP 90-beta ELISA Kit; HS90B_HUMAN ELISA Kit; HSP 84 ELISA Kit; HSP 90 ELISA Kit; HSP 90 b ELISA Kit; HSP 90b ELISA Kit; HSP84 ELISA Kit; HSP90 BETA ELISA Kit; hsp90ab1 ELISA Kit; HSP90B ELISA Kit; HSPC2 ELISA Kit; HSPCB ELISA Kit
缩写:HSP90AB1
Uniprot No.:P08238
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:1.56 ng/mL-100 ng/mL
灵敏度:0.39 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Neuroscience
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HSP90AB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 96 | |||||
Range % | 91-102 | ||||||
1:2 | Average % | 104 | |||||
Range % | 99-108 | ||||||
1:4 | Average % | 92 | |||||
Range % | 85-96 | ||||||
1:8 | Average % | 98 | |||||
Range % | 95-104 |
回收率:
The recovery of human HSP90AB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 93 | 86-96 | |||||
EDTA plasma (n=4) | 107 | 103-111 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected1002.7842.6482.7162.604502.0762.0092.0431.931251.3651.3371.3511.23912.50.7770.7540.7660.6546.250.4320.4160.4240.3123.120.2810.2740.2780.1661.560.1960.1880.1920.08000.1130.1110.112 |
货期:3-5 working days
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