首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：大鼠热休克蛋白27(HSP-27)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPB1含量。HSPB1为热休克蛋白B1，它在细胞应对应激，如高温、氧化应激等过程中发挥重要作用。研究其机制有助于了解细胞应激反应、凋亡调控等生物学过程，也为相关疾病如神经退行性疾病、癌症等的治疗提供潜在靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于科研领域中对大鼠模型在热应激、神经退行性疾病、代谢性疾病或组织损伤修复等实验条件下的 HSP - 27 动态变化研究，为探索其分子机制及调控网络提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hspb1 ELISA Kit; Hsp27 ELISA Kit; Heat shock protein beta-1 ELISA Kit; HspB1 ELISA Kit; Heat shock 27 kDa protein ELISA Kit; HSP 27 ELISA Kit
缩写：HSPB1
Uniprot No.：P42930
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 93-102 | ||||||
| 1:2 | Average % | 105 | |||||
| Range % | 100-113 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
回收率：
| The recovery of rat HSP-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 85 | 80-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 502.3612.2932.3272.126 251.6741.5921.6331.432 12.51.0021.0561.0290.828 6.250.7290.7040.7170.516 3.120.4470.4710.4590.258 1.560.3350.3180.3270.126 0.780.2780.2690.2740.073 00.1960.2050.201 | |||||||
货期：3-5 working days
×
