首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠ICOS配体(ICOSLG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ICOSLG含量。ICOSLG（诱导型T细胞共刺激配体）是一种跨膜糖蛋白，属于B7配体家族，广泛表达于多种抗原呈递细胞和非淋巴细胞。它作为T细胞特异性细胞表面受体ICOS的配体，在T细胞的活化、增殖、分化和细胞因子产生中起重要作用，同时促进B细胞分泌抗体。ICOSLG的研究机制涉及其在T/B细胞相互作用中的正反馈调节，以及与PI3K-AKT通路在肿瘤免疫治疗中的作用。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于免疫调节机制研究、疾病模型验证或药物干预实验等科研场景。通过检测不同生理病理条件下 ICOSLG 的表达动态，可为免疫微环境调控、细胞信号通路探索及治疗靶点开发提供可靠数据支持，满足免疫学基础研究与转化医学实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Icoslg ELISA Kit; B7h2 ELISA Kit; B7rp1 ELISA Kit; IcoslICOS ligand ELISA Kit; B7 homolog 2 ELISA Kit; B7-H2 ELISA Kit; B7-like protein Gl50 ELISA Kit; B7-related protein 1 ELISA Kit; B7RP-1 ELISA Kit; LICOS ELISA Kit; CD antigen CD275 ELISA Kit
缩写：ICOSLG
Uniprot No.：Q9JHJ8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of mouse ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.4172.5522.4852.32220002.1042.0762.0901.92710001.4501.4041.4271.2645000.7650.7230.7440.5812500.4390.4710.4550.2921250.3210.3420.3320.16962.50.2430.2290.2360.07300.1690.1570.163 | ||||||
货期：3-5 working days
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