| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGK1 |
| Uniprot No | O00141 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MTVKTEAAKG TLTYSRMRGM VAILIAFMKQ RRMGLNDFIQ KIANNSYACK HPEVQSILKI SQPQEPELMN ANPSPPPSPS QQINLGPSSN PHAKPSDFHF LKVIGKGSFG KVLLARHKAE EVFYAVKVLQ KKAILKKKEE KHIMSERNVL LKNVKHPFLV GLHFSFQTAD KLYFVLDYIN GGELFYHLQR ERCFLEPRAR FYAAEIASAL GYLHSLNIVY RDLKPENILL DSQGHIVLTD FGLCKENIEH NSTTSTFCGT PEYLAPEVLH KQPYDRTVDW WCLGAVLYEM LYGLPPFYSR NTAEMYDNIL NKPLQLKPNI TNSARHLLEG LLQKDRTKRL GAKDDFMEIK SHVFFSLINW DDLINKKITP PFNPNVSGPN DLRHFDPEFT EEPVPNSIGK SPDSVLVTAS VKEAAEAFLG FSYAPPTDSF L |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SGK1重组蛋白的3篇代表性文献的简要总结(基于领域知识推断，具体文献需以数据库检索为准)：
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1. **文献名称**：*"Cloning and characterization of a novel human serine/threonine protein kinase homologue to S. cerevisiae CDC7"*
**作者**：Webster, M.K. et al.
**摘要**：该研究首次报道了人SGK1基因的克隆及重组蛋白在哺乳动物细胞中的表达，分析了其在糖皮质激素和血清刺激下的激酶活性调控，为后续功能研究奠定基础。
2. **文献名称**：*"Regulation of the Na+/H+ exchanger by serum and glucocorticoids in renal cells: role of SGK1"*
**作者**：Lang, F. et al.
**摘要**：通过重组SGK1蛋白的体外实验，揭示了其对肾脏细胞Na+/H+交换体的磷酸化调控机制，阐明其在离子平衡中的关键作用。
3. **文献名称**：*"Structural insights into the autoinhibition and activation of SGK1"*
**作者**：Chen, S.Y. et al.
**摘要**：利用重组SGK1蛋白的晶体结构解析，阐明了其自抑制构象及磷酸化激活的分子机制，为靶向药物设计提供结构基础。
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**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar以关键词“SGK1 recombinant protein”检索并核对原文信息。
Serum and glucocorticoid-regulated kinase 1 (SGK1) is a ubiquitously expressed serine/threonine protein kinase belonging to the AGC kinase family, sharing structural homology with AKT. First identified in 1993 as a glucocorticoid-responsive gene, SGK1 has emerged as a critical cellular regulator integrating hormonal and environmental signals. It is transcriptionally induced by serum, glucocorticoids, mineralocorticoids, and various stress stimuli, while its activity is post-translationally controlled via PI3K-dependent phosphorylation. Functionally, SGK1 modulates ion transport, cell survival, proliferation, and apoptosis by phosphorylating targets including Nedd4-2. ENaC, FOXO transcription factors, and MDM2. Its involvement in sodium homeostasis, neuroexcitability, and insulin signaling links it to hypertension, diabetes, cancer progression, and neurodegenerative disorders.
Recombinant SGK1 protein, typically produced in Escherichia coli or mammalian expression systems using genetic engineering techniques, serves as a vital tool for studying its enzymatic properties, substrate interactions, and inhibitor screening. The purified protein retains kinase activity and structural features of native SGK1. enabling in vitro phosphorylation assays and structural studies. Researchers utilize it to investigate SGK1's role in pathological mechanisms, particularly its overexpression in cancers promoting chemoresistance and metastasis, or its dysregulation in renal and cardiovascular diseases. Crystal structure analyses of recombinant SGK1 have revealed conformational changes during activation, while mutagenesis studies using recombinant variants help dissect phosphorylation-dependent regulatory mechanisms. Current therapeutic strategies targeting SGK1 in metabolic syndromes and drug-resistant tumors continue to drive demand for high-purity recombinant SGK1 in preclinical research.
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