首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人艾杜糖硫酸酯酶(IDS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IDS含量。人艾杜糖硫酸酯酶（IDS）是一种溶酶体酶，参与硫酸皮肤素和硫酸乙酰肝素的降解，其活性对于防止这些物质在溶酶体内积聚至关重要。IDS缺乏会导致亨特综合征等溶酶体贮积症，通过基因治疗或酶替代疗法来提高IDS活性是治疗这些疾病的关键。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品广泛应用于代谢调控机制研究、溶酶体贮积症疾病模型构建、基因治疗药物效价评估等科研领域，实验数据稳定可靠，支持多样本类型的重复检测，为探索IDS相关分子通路及病理机制提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha L iduronate sulfate sulfatase ELISA Kit; Alpha-L-iduronate sulfate sulfatase ELISA Kit; AW214631 ELISA Kit; Ids ELISA Kit; IDS_HUMAN ELISA Kit; Iduronate 2 sulfatase 14 kDa chain ELISA Kit; Iduronate 2 sulfatase 42 kDa chain ELISA Kit; Iduronate 2 sulfatase ELISA Kit; Iduronate 2-sulfatase 14 kDa chain ELISA Kit; Iduronate sulfatase ELISA Kit; Idursulfase ELISA Kit; MPS2 ELISA Kit; RP23-29M4.1 ELISA Kit; SIDS ELISA Kit
缩写：IDS
Uniprot No.：P22304
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Tags & Cell Markers
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of human IDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-102 | ||||
| EDTA plasma (n=4) | 87 | 80-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2572.3572.3072.20110001.9761.8721.9241.8185001.5321.5781.5551.4492501.1721.1511.1621.0561250.7110.7280.7200.61462.50.4260.4340.4300.32431.250.2620.2700.2660.16000.1050.1060.106 | ||||||
货期：3-5 working days
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