首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人具有四肽重复序列1的干扰素诱导蛋白(IFIT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IFIT1含量。IFIT1，也称为干扰素诱导蛋白与四肽重复1，是一种在干扰素处理后被诱导产生的蛋白质，主要功能是抑制病毒复制和翻译起始。其研究机制涉及病毒或干扰素调控，通过介导蛋白质-蛋白质相互作用和形成多蛋白质复合物来执行抗病毒免疫和干扰素信号通路的调控。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于病毒感染机制研究、宿主免疫应答分析、炎症性疾病模型建立及抗病毒药物开发等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C56 ELISA Kit; G10P1 ELISA Kit; GARG 16 ELISA Kit; GARG16 ELISA Kit; IFI 56 ELISA Kit; IFI 56K ELISA Kit; IFI-56K ELISA Kit; IFI56 ELISA Kit; IFIT-1 ELISA Kit; Ifit1 ELISA Kit; IFIT1_HUMAN ELISA Kit; IFNAI1 ELISA Kit; Interferon alpha inducible protein (MW 56kD) ELISA Kit; Interferon induced 56 kDa protein ELISA Kit; Interferon induced protein 56 ELISA Kit; Interferon inducible mRNA 561 ELISA Kit; Interferon-induced 56 kDa protein ELISA Kit; Interferon-induced protein with tetratricopeptide repeats 1 ELISA Kit; ISG56 ELISA Kit; OTTHUMP00000020062 ELISA Kit; P56 ELISA Kit; RNM561 ELISA Kit
缩写：IFIT1
Uniprot No.：P09914
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IFIT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 93-103 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 97-108 | ||||||
回收率：
| The recovery of human IFIT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 96-105 | |||||
| EDTA plasma (n=4) | 89 | 83-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.8352.9862.9112.7705002.1832.2952.2392.0982501.3211.3971.3591.2181250.7020.7140.7080.56762.50.4530.4350.4440.30331.250.2770.2850.2810.14015.60.2290.2240.2270.08600.1400.1420.141 | |||||||
货期：3-5 working days
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