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产品描述：小鼠β干扰素(IFN-β/IFNB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的IFNB1含量。IFNB1即干扰素β-1，它是一种细胞因子。其背景在于具有抗病毒、抗增殖和免疫调节等多种生物学活性。研究机制方面，它可与细胞表面受体结合，激活信号转导通路，诱导下游抗病毒蛋白产生，从而发挥对病毒感染和一些免疫相关疾病的作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品可广泛应用于基础科研场景，如病毒感染模型中免疫应答动态监测、基因编辑动物IFN-β表达水平验证、免疫调节药物体外筛选评估以及炎症相关信号通路研究等。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ifnb1 ELISA Kit; Ifb ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
缩写：IFNB1
Uniprot No.：P01575
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-103 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 93-96 | ||||
| EDTA plasma (n=4) | 100 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5162.5682.5422.4375002.1152.1352.1252.0202501.4011.4351.4181.3131250.7610.7300.7460.64162.50.3430.3690.3560.25131.20.2420.2530.2480.14315.60.1790.1720.1760.07100.1040.1060.105 | ||||||
货期：3-5 working days
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