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产品描述：绵羊γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ ，是一种具有多种生物学活性的细胞因子。在免疫调节方面背景深厚，参与机体的抗病毒、抗肿瘤等免疫反应。其研究机制主要围绕调节免疫细胞功能，促进Th1细胞分化，增强巨噬细胞活性，激活自然杀伤细胞等。试剂盒检测范围为5 pg/mL-200 pg/mL，适用于科研场景下对免疫相关机制的探索；可用于评估绵羊在疫苗接种、寄生虫感染（如旋毛虫病）或细菌性疾病（如布鲁氏菌病）等实验模型中Th1型免疫反应的动态变化；适用于比较不同处理条件下免疫调节机制的研究；为畜牧科学、兽医学及比较免疫学领域的研究者提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFNG
Uniprot No.：P17773
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：5 pg/mL-200 pg/mL
灵敏度：2.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 95-102 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of sheep IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-95 | ||||
| EDTA plasma (n=4) | 86 | 83-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2002.7762.7982.787 1002.0882.0982.093 501.1641.1871.176 250.6750.6330.654 100.2640.2870.276 50.1250.1270.126 | ||||||
货期：3-5 working days
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