首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠α干扰素(IFN-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IFNA1含量。IFNA1即干扰素α1，是Ⅰ型干扰素家族成员。它具有抗病毒、免疫调节等功能。其研究机制主要是通过与细胞表面受体结合，激活下游信号通路，诱导多种抗病毒蛋白表达，从而发挥对病毒复制和传播的抑制作用，在抗病毒及免疫相关研究中受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外免疫学研究、病毒感染模型评估或药物干预实验中IFN-α表达水平的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit
缩写：IFN-α
Uniprot No.：P05011
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-91 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 99-108 | |||||
回收率：
| The recovery of rat IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.0872.0992.0931.916 10001.5951.5741.5851.408 5001.0871.0961.0920.915 2500.6720.6950.6840.507 1250.4910.4570.4740.297 62.50.3760.3510.3640.187 31.250.2870.2990.2930.116 00.1750.1780.177 | ||||||
货期：3-5 working days
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