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产品描述：豚鼠免疫球蛋白E(IgE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IgE含量。试剂盒检测范围为93.75 ng/mL-6000 ng/mL，在豚鼠模型中常用于研究过敏性疾病机制、药物疗效评估及免疫调节实验；科研人员可通过该产品探究豚鼠在过敏原暴露、免疫干预或感染性疾病过程中IgE的动态变化，为建立过敏模型、疫苗开发或免疫调控研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgE
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 ng/mL-6000 ng/mL
灵敏度：23.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-97 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 96-107 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of guinea pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-96 | ||||
| EDTA plasma (n=4) | 106 | 102-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected60002.7552.6542.7052.61530002.0281.9171.9731.88315001.1951.1741.1851.0957500.5690.5580.5640.4743750.3220.3120.3170.227187.50.2540.2430.2490.15993.750.1480.1440.1460.05600.0900.0890.090 | ||||||
货期：3-5 working days
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