首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：绵羊免疫球蛋白E(IgE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IgE含量。IgE是一种在哺乳动物体内发现的抗体，含量极低，主要由黏膜下淋巴组织中的效应B细胞合成。它是一种亲细胞抗体，可引起I型变态反应，如过敏。研究显示，IgE与高亲和力受体FcεRI相互作用是过敏反应的核心环节，其激活机制涉及FcεRI从二聚体转变为单体，从而激活下游信号通路。试剂盒检测范围为125 ng/mL-8000 ng/mL，适用于科研领域对绵羊免疫状态评估、过敏性疾病机制研究或寄生虫感染模型的免疫指标分析，为畜牧学、兽医学及比较免疫学研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgE
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：125 ng/mL-8000 ng/mL
灵敏度：31.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of sheep IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-102 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected80002.6322.5122.5722.42840002.0741.9742.0241.88020001.3711.2711.3211.17710000.8150.8050.8100.6665000.4700.4600.4650.3212500.3480.3380.3430.1991250.2380.2290.2340.09000.1440.1430.144 | ||||||
货期：3-5 working days
×
