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产品描述：牛胰岛素样生长因子II(IGF2)酶联免疫试剂盒，双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IGF2含量。IGF2即胰岛素样生长因子 2，在胚胎发育、细胞增殖与分化等过程中发挥关键作用。其异常表达与多种肿瘤发生发展相关。研究聚焦于其信号通路机制，以及与其他因子相互作用，旨在探索以 IGF2 为靶点的肿瘤诊断和治疗新策略。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，该产品适用于畜牧科研中生长调控机制研究、营养代谢评估，以及基础医学领域对IGF2生物学功能的探索，例如在牛胚胎发育模型、肌肉生长研究或代谢综合征动物模型中的应用。其宽泛的检测范围尤其适合动态监测IGF2水平变化，为深入解析该因子在生理及病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
缩写：IGF2
Uniprot No.：P07456
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 100-114 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 101-106 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 84-91 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-96 | ||||||
回收率：
| The recovery of bovine IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 88 | 85-91 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected20001.9581.9521.9551.85810001.1661.0761.1211.0245000.6710.6540.6630.5662500.3980.3890.3940.2971250.2630.2540.2590.16262.50.1770.1690.1730.07631.250.1350.1290.1320.03500.0960.0970.097 | |||||||
货期：3-5 working days
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